Cell Biology Biol-157L

Joint Science Department

of the

Claremont Colleges

Professor Ernest Y. Kwok

Mead Hall Room 131i

ekwok@jsd.claremont.edu

(909) 607-2774

Office hours: Tuesday & Thursday 1:30-3:30pm  

 

Lectures: Monday & Wednesday 12:00pm-1:15pm Scripps Humanities Room 104

Laboratory: Monday 1:30pm-5:15pm Keck Science Center Room B32

Textbook: The World of the Cell 6th Edition.  Becker Kleinsmith & Hardin.  Pearson-Benjamin Cummings: 2006.  ISBN: 0805346805

 

Class Syllabus

Monday Lecture

Monday Lab

Wednesday Lecture

 

 

1/23  Cell theory and Introduction to course

Assignment (pdf)

1/28 Proteins and analysis- Notes (pdf)

Group presentation assignment (pdf)

Group presentation topics (pdf)

1/28 Light Microscopy

Lab syllabus (pdf)

Microscopy project (pdf)

1/30 Protein Analysis

 

 

2/4 Fractionation & Fluorescence Microscopy

2/4 Image collection

2/6 Group Presentations: Endosymbiosis (pdf) & Chloroplast Protein targeting (pdf)

2/11 Group Presentations: Endoplastmic Retuculum (pdf) & Golgi/Glycosylation (pdf)

Chloroplast isolation prelab due at beginning of lecture

 

2/11 Chloroplast isolations (protocol & prelab)

Chloroplast Glycoprotein Overview (pdf)

 

2/13 Group Presentations: Protein targeting to Nucleus, Mitochondria, Peroxisomes (pdf)

Figure analysis assignment

Microscopy first draft due

2/18 Paper analysis

Figure analysis HW due

2/18 SDS-PAGE and blotting (protocol)

2/20 Paper Analysis

Microscopy final draft due

2/25 The Cytoplasm 2/25 Western detection (protocol & prelab)

2/27 Independent Lab Project Discussion

1st Midterm Exam assigned Figures Solutions

3/3 Actin Microfilaments

Reading and HW Assignment Solutions

3/3 Independent Project 1/2

 

3/5 Actin Binding Proteins

1st Midterm Exam due

3/10 Myosins and Motility

 

3/10 Independent Project 2/2

Lab Report Instructions

3/12 Introduction to Listeria & Intermediate Filaments and Extracellular Matrix

Microfilament HW due

3/17 Spring Break 3/17 Spring Break 3/19 Spring Break

3/24 Apoptosis Lecture

 

3/24 Apoptosis Introduction (protocol)

Project Overview

3/26 Microtubules Discussion

Assignment

3/31 Paper Analysis

Figure Analysis Due

 

3/31 Apoptosis Induction (prototcol)

 

 

4/2 Paper Analysis

Figure Analysis Due

Chloroplast/Glycosylation Lab Report Draft due

4/7 The Nucleus & Chromosomes

Prelab Due

4/7 Apoptosis Analysis (protocol & prelab)

 

4/9 Discuss Apoptosis Data and Independent Projects

2nd Midterm Exam assigned Figures

4/14 Cell Cycle

Discuss Independent Projects

 

4/14 Independent Project 1/3

 

 

4/16 Mitosis

Chloroplast/Glycosylation Lab Report Final due

2nd Midterm Exam due Solutions

4/21 Paper Analysis Assignment

Paper Questions due

4/21 Independent Project 2/3

 

4/23 Paper Analysis

 

4/28 Apoptosis/Cancer/Stem Cells

Reading Assignment

 

4/28 Independent Project 3/3

 

 

4/30 Cloning Ethics

Reading and HW Assignment

Final Exam assigned Figure A

5/5 Presentations 5/5 Presentations

5/7 Senior Final Due

    5/14 Regular Final Due

 

Reading Links

Honnold Library Electronic Journals Collection

Pubmed Database

“Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast.”

-Villarejo et al. (2005) Nature Cell Biology 7 : 1224-1231.

“Fascin-mediated propulsion of Listeria monocytogenes independent of frequent nucleation by the Arp2/3 complex.”

-Brieher et al. (2004) The Journal of Cell Biology 165(2):233-242

“Apoptosis: controlled demolition at the cellular level.”

Taylor et al. (2008) Nature Reviews Molecular Cell Biology (9): 231-241

"Differentiation of Cytoplasmic and Meiotic Spindle Assembly MCAK Functions by Aurora B-dependent Phosphorylation"

-Ohi et al. (2004) Molecular Biology of the Cell 15(6): 2895-2906

“Understanding Stem Cells: An Overview of the Science and Issues from the National Academies.”

-Human Embryonic Stem Cell Research Advisory Committee (2006)

“Human Cloning and Human Dignity: An Ethical Inquiry.”

-The President's Council on Bioethics (2002)

Sequence Analysis Tools

ExPASy proteomics server

Evaluations

Audience evaluations of group presentations (pdf)

Peer evaluations of group assignments (pdf)

Peer evaluations of laboratory projects (pdf)